Journal: ACS chemical biology

Article Title: A Chemical Biology Solution to Problems with Studying Biologically Important but Unstable 9-O-Acetyl Sialic Acids

doi: 10.1021/acschembio.6b00928

Figure Lengend Snippet: Sialoglycan microarray binding specificity studies of human Siglec-9 (hSiglec-9-Fc, hSiglec-9), porcine torovirus hemagglutinin-esterase (PToV), and bovine coronavirus hemagglutinin-esterase (BCoV) (both PToV and BCoV were mutated to ablate their esterase activity) towards Neu5Ac9NAcα3Galβ4GlcβProNH2 (black columns), Neu5,9Ac2α3Galβ4GlcβProNH2 (gray columns), and Neu5Acα3Galβ4GlcβProNH2 (white columns) without esterase treatment (A) or treated with esterase active PToV (B).

Article Snippet: Glycan Microarray Screening Glycan microarrays were fabricated using epoxide-derivatized slides (Corning by Thermo Fisher Scientific) and Arrayit SpotBot® Extreme Microarray Spotter as previously described.

Techniques: Microarray, Binding Assay, Activity Assay

Journal: Biotechnology Reports

Article Title: High-throughput microarray for antimicrobial susceptibility testing

doi: 10.1016/j.btre.2017.10.004

Figure Lengend Snippet: High-throughput AST chip. (A) A schematic of high-throughput antimicrobial susceptibility testing using AST-Chip. Antimicrobial susceptibility testing using the chip can be conducted in 12 h; Microarray micrograph of S. aureus cultures formed on hydrogel spots of AST-Chip after 12–18 h. The spots are interspaced at 1 mm with a spot diameter of 0.5 mm; The nano-biofilms analyzed using FUN-1 is demonstrated in green color; (B) BacLight live/dead assay shows metabolically active cells in yellow-orange color, present as colonies. The scale bar is 100 μm; (C) SEM micrograph of S. aureus nano-biofilm colonies. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)

Article Snippet: Any subsequent dilutions needed for the antimicrobial susceptibility assays were made in PBS, mixed in a suspension of 0.5% alginate, and optimized media, and 50 nL of this mixture was spotted using a non-contact microarray spotter (Omnigrid Micro, Digilab Inc., Holliston, MA) on modified glass slides.

Techniques: High Throughput Screening Assay, Microarray, Live Dead Assay, Metabolic Labelling

Journal: Biotechnology Reports

Article Title: High-throughput microarray for antimicrobial susceptibility testing

doi: 10.1016/j.btre.2017.10.004

Figure Lengend Snippet: Antimicrobial susceptibility profile of S. aureus . AST of methicillin against wild-type (UAMS-1WT) and community-acquired methicillin-resistant Staphylococcus aureus (CA-MRSA). (A) Microarray scanner image of AST-Chip with S. aureus cultures exposed to different concentrations of methicillin [MET]. The viability is indicated by the fluorescence intensity of the spots. (B) Scanning electron micrographs of S. aureus biofilms that are untreated (top) or treated with 10 μg/ml MET (bottom); (C) Dose-response profile of S. aureus cultures obtained using AST-Chip.

Article Snippet: Any subsequent dilutions needed for the antimicrobial susceptibility assays were made in PBS, mixed in a suspension of 0.5% alginate, and optimized media, and 50 nL of this mixture was spotted using a non-contact microarray spotter (Omnigrid Micro, Digilab Inc., Holliston, MA) on modified glass slides.

Techniques: Microarray, Fluorescence